Specifically designed for advanced multiphoton microscopy, InSight® X3™ and InSight® X3+™ ultrafast tunable lasers are built on MKS Spectra-Physics’ industry leading InSight platform, with hundreds of peer-reviewed publications, the largest installed base in the market and demonstrated high reliability.
Based on patented technology, InSight features a broad 680 nm to 1300 nm continuous, gap-free tuning curve from a single beam with nearly double the tuning range of legacy Ti:Sapphire lasers. InSight is designed with the peak of the tuning curve centered in the 900 to 1100 nm region to excite the most common green and red fluorophores including GFP, RFP, GCaMP, jRGECO, and mCherry.
InSight X3+ delivers 50% greater power across the tuning range for deeper imaging and more complex experiments and additionally provides the highest powers at the longest wavelengths of 1280 to 1300 nm for excitation of far red fluorophores such as Alexa-680.
With MKS Spectra-Physics’ integrated and patented DeepSee™ dispersion pre-compensation module, the short femtosecond pulses are optimally delivered through a microscope to the sample providing maximum fluorescence and penetration depth. InSight has exceptional beam pointing stability, beam quality, output power stability and noise, with fast wavelength tuning.
When equipped with the fixed 1045 nm dual beam option, the InSight platform fully supports the diverse needs of multimodal imaging. The two synchronized output beams enable easy simultaneous imaging of various fluorescence proteins, genetically encoded calcium indicators, second and third harmonic generation, and advanced imaging techniques such as CARS and SRS. For these advanced techniques, InSight can be combined with the Newport Timing and Recombination Unit (TRU) or the Spectral Focusing Timing and Recombination Unit (SF-TRU).
High Power Across Entire Tuning Range With the Industry’s Highest Power at the Multiphoton Imaging Wavelengths Between 900 and 1300 nm
Average Output Power: >1.0 to >3.5 W
Field Proven With the Largest Installed Base in the Market, Hundreds of Peer-reviewed Publications and Demonstrated High Reliability
Broad Tuning Range for Maximum Flexibility: 680 to 1300 nm
Tuning Speed: >50 nm/s
Dual Output at 1045 nm With Optional Pre-compensation for Simultaneous Two Color Excitation
Pulse Width: <120 to <200 fs
Noise: <0.25%, <0.5%
Pre-compensation Dispersion Range: 0 to -40,000 fs²