LAMPPY Optical DNA Rapid Detection System For Pathogens

AMS item number: SW11790

High-intensity LED Array; 478 nm; Detection Range 500-595 nm; Optical Dynamic Range 60 dB;... more

Product information "LAMPPY Optical DNA Rapid Detection System For Pathogens"

High-intensity LED Array; 478 nm; Detection Range 500-595 nm; Optical Dynamic Range 60 dB; Electrical Dynamic Range 120 dB; Acquisition Frequency 10 s-2 min

With LAMPPY™, OZ Optics provides an exceptionally affordable, yet reliable optical DNA detection system based on isothermal techniques such as "Loop-mediated Isothermal Amplification" (LAMP). Much like "Polymerase Chain Reaction" (PCR) amplification, LAMP is a highly sensitive method of DNA detection that creates billions of copies of a target DNA from a minute amount of sample RNA or DNA. However, unlike PCR, LAMP applies an isothermal process that does not require expensive and complex equipment, nor time consuming thermal cycling. It is a more cost-effective and rapid system.

LAMP is especially ideal for rapid diagnosis of pathogens or infectious diseases, with results usually within 30 minutes and for high viral loads less than 20 minutes. The LAMPPY™ system uses an array of high powered LEDs, photodiodes and filters for real-time quantitative detection. The system can be paired with an external battery for a compact, field-deployable diagnostic instrument.

Samples to be tested are first mixed with a solution of primers, enzymes, an intercalating dye, and several other components. This mixture is then dispensed into standard 0.1 mL PCR tubes with flat caps, commonly known as low-profile PCR tubes. Acceptable sample volumes can be between 5 µl and 125 μl (10 to 30 μl recommended).

The instrument can work with many different solution kits from third parties for different pathogens. For that reason, OZ Optics considers it to be a universal DNA/RNA detection system. The standard system utilizes an excitation diode/filter combination emitting at 450 nm to 488 nm and a filter/detector combination for 500 nm to 595 nm light. This setup is compatible with many popular fluorescent dyes, including SYTO 9, SYBR Green and EvaGreen dyes. Solution kits using these or similar dyes can work with the instrument as-is. However, if the user wants to work with different dyes that utilize different wavelengths, AMS Technologies can provide filter blocks with different filter combinations. The filter blocks can be exchanged by the user, making the instrument extremely versatile and easy to service with downtime reduced to a minimum.

The accompanying software is intuitive and easy to use, providing quantitative measurements in real-time while allowing fast and easy setup of assays, protocols, and experiments that can be saved and reused. It will automatically detect attached instruments for quick setup and can run multiple instruments simultaneously on one computer. Data can be exported as CSV, XML, JSON, or PNG. Standard communication is via a USB cable, wireless communication via Wi-Fi will also be offered.

Key Features:

Use to Detect Viral, Fungal, and Bacterial DNA/RNA Including Covid-19, SARS, Ebola, Cholera, Salmonella, etc.
Rapid DNA/RNA Detection (from 20 min)
Highly Sensitive and Specific Detection of Low Viral Levels
Intuitive Software Displays Real-time Data During Testing
Melt Analysis Available With the Included Software
Lid Heater Prevents Evaporation and Condensation
Compact Modular Design Allows for Easy Cleaning and Maintenance
Pair With an External Battery for a Portable and Field-deployable System
Test up to 8 Samples Simultaneously (Higher Throughput Systems With up to 96 Samples Available Upon Request)
Wireless Communication to Computers and Smartphones (coming soon)
A Fraction of the Cost of qPCR-based Systems
Private Labelling for Volume OEM Applications on Request
Electrical Dynamic Range: ~120 dB
Optical Dynamic Range: ~60 dB
Excitation Wavelength: 478 nm, FWHM = 20 nm (Typical)
Detection Wavelength Range: 500 nm to 595 nm
Temperature Range: Adjustable From Ambient to +94°C (Block) and +99°C (Lid) in 1°C Increments
Heating Ramp Rate: ~9.6 s/°C
Standard Throughput / Test Wells: 8
Acquisition Frequency: 10 s to 2 min
Light Source: High-intensity LED Array
Detector: Photodiode Array
Interface: USB Type B
Power Supply: 100-240 VAC Input, 12 VDC Output, <50 W
Dimensions: 163 x 104 x 127 mm
Weight: 1.67 kg

Applications: Screening and Diagnosis of Infection Disease; Food and Water Testing for Microbes; Molecular Biology Research; Dry Block Incubator